Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse
SIGMA/D5167 - clone 70.1, ascites fluid
Synonym: Anti-CILD1; Anti-DIC1; Anti-ICS1; Anti-PCD
MDL Number: MFCD02262824
Product Type: Chemical
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in Mouse, Clone: 70.1 (Cat. No. D5167). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 70.1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | marsupial, hamster, human, Indian muntjac (Muntiacus muntjac vaginalis), chicken, rat, frog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1:2,000 using a chick brain extract | |
| UniProt accession no. | Q9UI46  |
| Application: | Monoclonal Anti-Dynein (Intermediate Chain) antibody produced in mouse has been used in: • perturbation assays • western blotting • immunofluorescence microscopy • enzyme linked immunosorbent assay (ELISA) • dot blot • immunocytochemistry • immunopurification |
| Biochem/physiol Actions: | Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Dynein is a multimeric motor protein which transports membrane-bound vesicles, proteins and organelles towards the negative end of microtubules (retrograde transport). It has a crucial role in cytoplasmic motility, chromosomal movement and the organization of the Golgi apparatus. Monoclonal anti-dynein (Intermediate Chain) antibody can be used for localization of dynein intermediate chain using different immunochemical assays like ELISA, immunoblotting and dot blot. Mouse anti- dynein (Intermediate Chain) antibody reacts specifically with dynein of frog, hamster (CHO cell line), chicken, rat, marsupial (rat kangaroo, PtK1 cell line), Indian muntjac (Muntiacus muntjac vaginalis) and human (HeLa,MCF-7). |
| General description: | Monoclonal anti-Dynein (Intermediate Chain) (mouse IgM isotype) is derived from the 70.1 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with cytoplasmic dynein purified from chick brain. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (approximately 450 kDa), three intermediate chains (70- 125 kDa), and at least four light chains (15-25 kDa). Dynein intermediate chain (ICs) is a component of dynein multisubunit. Dynein intermediate chain interacts with dynactin through its N-terminal region. |
| Immunogen: | cytoplasmic dynein from chicken embryo brain |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |