Deoxyribonuclease I bovine
SIGMA/D5319 - recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein
Synonym: DNAse I; Deoxyribonucleate 5′-oligonucleotido-hydrolase
MDL Number: MFCD00130918
Product Type: Chemical
SDS-PAGE Deoxyribonuclease I Bovine (Cat. No. D5319) was assessed on SDS-PAGE.
| application(s) | diagnostic assay manufacturing |
| assay | ≥95% |
| biological source | bovine |
| foreign activity | RNAse and protease, free |
| form | buffered aqueous glycerol solution |
| mol wt | ~39 kDa |
| Quality Level | 200  |
| recombinant | expressed in Pichia pastoris |
| shipped in | dry ice |
| specific activity | ≥5,000 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA extraction: suitable |
| Application: | Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease. |
| Application: | DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands. |
| Application: | Used for the removal of DNA from protein samples. |
| Biochem/physiol Actions: | Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved. |
| Biochem/physiol Actions: | DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. |
| Features and Benefits: | • RNA purification by removing DNA • Prepare DNA for nick translation1 • Footprinting assays to determine DNA-protein interactions2 |
| Other Notes: | One unit will produce a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C |
| Physical form: | Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol |
| Preparation Note: | Produced without using any animal cells or animal derived materials. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% |
| activity | specific activity: ≥5,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.21.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |