Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit
SIGMA/D5567 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-H3K9me2
MDL Number: MFCD06798437
Product Type: Chemical
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in Rabbit (Cat. No. D5567). The antibody was developed using 1:50,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. 1:2,000 antibody 4. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 17 kDa |
| shipped in | dry ice |
| species reactivity | Drosophila, bovine, chicken, Arabidopsis thaliana, Caenorhabditis elegans, human, mouse, frog, rat |
| storage temp. | −20°C |
| target post-translational modification | dimethylation (Lys9) |
| technique(s) | ChIP: suitable |
| microarray: suitable | |
| western blot: 1:1,000-1:2,000 using whole extract of human epitheloid carcinoma HeLa cell line | |
| UniProt accession no. | P68431   |
| Application: | Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit has been used in immunoblotting and chromatin immunoprecipitation assay. |
| Biochem/physiol Actions: | ChIP validated |
| Biochem/physiol Actions: | Mono- and dimethylation of H3 at Lys9 are intrinsically linked to epigenetic silencing and heterochromatin assembly. Methylation of H3 at Lys9 generates a binding site for HP1 proteins, a family of heterochromatic adaptor proteins implicated in both gene silencing and in the organization of higher order chromatin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone methylation is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis and heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation. SET domain-, site-specific histone methyltransferases (HMTases) are involved in methylation of Lys9 in histone 3. |
| Immunogen: | synthetic methylated peptide corresponding to amino acids 5-13 (diMe-Lys9) of human histone H3, conjugated to KLH. This sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, C. elegans, tetrahymena, and Arabidopsis thaliana histone H3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |