3,3′-Diaminobenzidine tetrahydrochloride hydrate

SIGMA/D5637 - ≥96%

Synonym: (1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate

CAS Number: 868272-85-9
EC Number: 231-018-9
MDL Number: MFCD08273058
Linear Formula: C₁₂H₁₄N₄ · 4HCl · xH₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-D5637-1G	1 g	$49.50 1/EA	Add To Favorites
45-D5637-5G	5 g	$166.00 1/EA	Add To Favorites
45-D5637-10G	10 g	$291.00 1/EA	Add To Favorites
45-D5637-25G	25 g	$652.00 1/EA	Add To Favorites
45-D5637-50G	50 g	$1080.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5637-25G.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing hematology histology
assay	≥96%
color	white to light brown, and Faint Red and Faint Grey to Light Grey
form	powder, crystals or chunks
InChI	1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
InChI key	DXWSCXIZIHILNP-UHFFFAOYSA-N
mp	280 °C
Quality Level	200
SMILES string	O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
solubility	H₂O: 50 mg/mL
storage temp.	−20°C
technique(s)	titration: 96-104% (with NaOH)

Application:	3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).
Biochem/physiol Actions:	In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.
General description:	3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.
Packaging:	1, 5, 10, 25, 50 g in glass bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H341 - H350
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	Xn
Risk Statements	45-22-36-68
Safety Statements	53-26-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Purity	≥96%
mp	280 °C
Storage Temp.	−20°C
UNSPSC	12171500

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