Dulbecco’s Modified Eagle’s Medium - high glucose
SIGMA/D5648 - With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym: DME; Dulbecco′s Modified Eagle′s Medium - high glucose; DME; DMEM
Product Type: Chemical
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| components | phenol red: yes L-glutamine: yes NaHCO3: no HEPES: no glucose: high sodium pyruvate: no |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of |
| Application: | Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue |
| Preparation Note: | Formulated to contain 13.4 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 3.7 g/L sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |