Diethyl pyrocarbonate
SIGMA/D5758 - 96% (NT)
Synonym: DEP; DEPC; Diethyl dicarbonate; Diethyl oxydiformate; Ethoxyformic acid anhydride
CAS Number: 1609-47-8
Empirical Formula (Hill Notation): C6H10O5
Molecular Weight: 162.14
EC Number: 216-542-8
MDL Number: MFCD00009106
Linear Formula: O(COOC2H5)2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5758-25ML.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5758-50ML.
| assay | 96% (NT) |
| biological source | synthetic (microbial fermentation/ cell culture) |
| bp | 93-94 °C/18 mmHg (lit.) |
| density | 1.101 g/mL at 25 °C (lit.) |
| form | liquid |
| InChI | 1S/C6H10O5/c1-3-9-5(7)11- |
| InChI key | FFYPMLJYZAEMQB-UHFFFAOYSA |
| Quality Level | 200  |
| refractive index | n |
| shipped in | wet ice |
| SMILES string | CCOC(=O)OC(=O)OCC |
| storage temp. | 2-8°C |
| Application: | Diethyl pyrocarbonate has been used in PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases. It is also used for Dot blot hybridization, to dilute total RNA isolated from different micro-organisms. |
| Application: | Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. |
| Biochem/physiol Actions: | DEPC is a potent nuclease inhibitor.0.1% solution of DEPC is used to inactive RNase. It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose and time dependent manner and increases the Ca2+ permeability of SR vesicles. |
| Biochem/physiol Actions: | Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation. |
| General description: | Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It decomposes at 155°C, to ethanol and carbon dioxide in aqueous solution. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. |
| Packaging: | 5, 25, 50, 100 mL in glass bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P301 + P312 + P330 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 156.2 °F - closed cup |
| Flash Point(C) | 69 °C - closed cup |
| Purity | 96% (NT) |
| bp | 93-94 °C/18 mmHg (lit.) |
| Density | 1.101 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |