Dextranase from Penicillium sp.
SIGMA/D5884 - lyophilized powder, 10-25 units/mg solid
Synonym: 1,6-α-D-Glucan 6-glucanohydrolase
CAS Number: 9025-70-1
EC Number: 232-803-9
MDL Number: MFCD00130940
Product Type: Chemical
Dextran is composed of approximately 95% α-D-(1-6) linkages. The remaining α(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000 dextran molecules exhibit the properties of an expandable coil. At MWs below 2,000 dextran is more rod-like.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D5884-5KU
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 10-25 units/mg solid |
| storage temp. | 2-8°C |
| Analysis Note: | Crude |
| Application: | Dextranase from Penicillium sp. has been used in a study to assess a new method of synthesizing biopolymeric affinity ligands. Dextranase from Penicillium sp. Has also been used in a study to investigate the cloning and sequencing of a dextranase-encoding cDNA from Penicillium minioluteum. |
| Application: | Dextranase from Sigma has been used for the hydrolysis of carbohydate polymers, during the study of polysaccharide synthesis by Phanerochaete chrysosporium. It has also been used in the synthesis of new enzymatically degradable thermo-responsive nanogels. |
| Biochem/physiol Actions: | An endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production. |
| Other Notes: | One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 5000 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 10-25 units/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |