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3,3′-Diaminobenzidine tetrahydrochloride

SIGMA/D5905 - tablet, 10 mg substrate per tablet

Synonym: 3,3′,4,4′-Biphenyltetramine tetrahydrochloride; 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride; DAB

Empirical Formula (Hill Notation): C12H14N4 · 4HCl
Molecular Weight: 360.11
EC Number: 231-018-9
MDL Number: MFCD00012969
Linear Formula: (NH2)2C6H3C6H3(NH2)2 · 4HCl
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D5905-50TAB 50 tabs
 $428.00
1/EA		 Add To Favorites
45-D5905-100TAB 100 tabs
 $732.00
1/EA		 Add To Favorites
45-D5905-5X100TAB 100 tabs
 $2770.00
5/EA		 Add To Favorites

 

form tablet
InChI 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
InChI key KJDSORYAHBAGPP-UHFFFAOYSA-N
Quality Level 200 
SMILES string Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
solubility water: 1 tablet/15 mL (may contain small insoluble particles)
storage temp. −20°C
Application: 3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.
Application: Reagent for spectrophotometric determination of selenium.
Biochem/physiol Actions: 3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.
Biochem/physiol Actions: Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.
Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research 
Preparation Note: Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.
Symbol GHS08  GHS08
Signal word Danger
Hazard statements H341 - H350
Precautionary statements P201 - P308 + P313
Hazard Codes T
Risk Statements 45-68
Safety Statements 53-45
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352204

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