pUC18 DNA Hae III Digest
SIGMA/D6293 - for DNA electrophoresis
Product Type: Chemical
D 6293 on a 4-20% PAGE gel
Representative product in the bag
| form | liquid |
| Quality Level | 100  |
| 200 |
|
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | 200 uses (0.1 mg size) |
| 40 uses (20 μg size) |
| Application: | pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen. |
| Application: | Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. |
| General description: | Sigma’s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
| Other Notes: | Contains 11 fragments, 11–587 bp |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 2.5%. |
| Other Notes: | Sigma’s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA. |
| Preparation Note: | Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |