Anti-DCP2 (C-terminal) antibody produced in rabbit
SIGMA/D6319 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DCP2 decapping enzyme homolog (S. cerevisiae); Anti-NUDT20; Anti-Nucleleoside diphosphate-linked moiety X motif 20; Anti-Nudix motif 20
Product Type: Chemical
Immunofluorescence Localization of DCP2 in cytoplasmic processing bodies (PB) in NIH-3T3 cells. Cells were stained with 5 μg/mL Rabbit Anti-DCP2 (Cat. No. D6319) followed by Goat Anti-Rabbit IgG, Cy3 conjugate. The cells were counterstained with DAPI (blue) to stain nuclei (x20 magnification).
Immunofluorescence Anti-DCP2: Cat. No. D6319: Immunofluorescence of HUVEC cells using Anti-DCP2 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Immunofluorescence Graph Anti-DCP2: Cat. No. D6319: Intensity analysis of Anti-DCP2 staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
Immunoblotting
Lysates from various cell lines were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-DCP2 (C-terminal) (Cat. No. D6139), The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lysates from the following cell lines were loaded:
Lanes: 1. K-562; 2. Jurkat; 3. A431; 4. MCSF; 5. 293-T; 6. 3T3; 7. C2; 8. RAT1; 9. PC12; and 10. A549
Lysates from various cell lines were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-DCP2 (C-terminal) (Cat. No. D6139), The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lysates from the following cell lines were loaded:
Lanes: 1. K-562; 2. Jurkat; 3. A431; 4. MCSF; 5. 293-T; 6. 3T3; 7. C2; 8. RAT1; 9. PC12; and 10. A549
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 celle |
| indirect immunofluorescence: suitable | |
| western blot: 2-4 μg/mL using lysaes of K-562 and Rat1 cells | |
| UniProt accession no. | Q8IU60  |
| Application: | Anti-DCP2 (C-terminal) antibody has been used in immunocytochemistry and image correlation analysis. It may also be used in immunoblotting. |
| Application: | Anti-DCP2 antibody produced in rabbit is suitable for indirect immunofluorescence at a working concentration of 2-5μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP2 and western blot analysis at a working concentration of 2-4μg/mL using lysates of K-562 and Rat1 cells. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if . |
| Biochem/physiol Actions: | Dcp2 is an RNA binding protein and can cleave only a cap structure that is linked to an RNA moiety, suggesting that Dcp2 can differentially associate with specific mRNAs. Dcp2 cleaves the m7G mRNA cap in the 5’ to 3’ mRNA decay pathway, in association with Dcp1 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5’ mRNA. Dcp2 is the catalytic subunit, and the mRNA is degraded by the major cytoplasmic 5′ to 3′ exonuclease XRN1. The enzymatic activity of DCP2 is critically dependent on the DCP1 subunit in vivo. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DCP2 (C-terminal) is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end. |
| General description: | Dcp2 colocalizes with Dcp1 in distinct cytoplasmic foci along with other proteins involved in the 5’ to 3’ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. hDCP2 contains a highly conserved Nudix (nucleoside diphosphate linked to an X moiety) motif critical for the decapping activity. |
| Immunogen: | synthetic peptide corresponding to amino acids 406-420 of human DCP2, conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |