Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham
SIGMA/D6434 - With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym: DME/Nutrient Mixture F-12 Ham; DMEM Hams F12; DMEM Hams F12; DME/F-12, 1:1 mixture
MDL Number: MFCD00217417
Product Type: Chemical
| components | L-glutamine: no phenol red: no NaHCO3: yes HEPES: 15 mM sodium pyruvate: 0.055 g/L glucose: 3.15 g/L |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture: • bovine endometrial epithelial cells • cancer stem cells • breast cancer cell line |
| General description: | Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture. |
| Preparation Note: | Supplement with 0.365 g/L |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |