JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR
SIGMA/D6442 - Ready-to-use 2x mix for qPCR with ROX
Synonym: Taq polymerase mix
Product Type: Chemical
Representative kit contents
| application(s) | agriculture |
| color | colorless |
| compatibility | for use with ABI 5700 |
| for use with ABI 7000 | |
| for use with ABI 7300 | |
| for use with ABI 7500 | |
| for use with ABI 7500 Fast | |
| for use with ABI 7700 | |
| for use with ABI 7900 | |
| for use with ABI 7900 Fast | |
| for use with ABI 7900 HT | |
| for use with ABI StepOne | |
| for use with ABI StepOnePlus | |
| for use with ABI ViiA 7 | |
| for use with Bio-Rad CFX384 | |
| for use with Bio-Rad CFX96 | |
| for use with Bio-Rad MiniOpticon | |
| for use with Bio-Rad MJ Chromo4 | |
| for use with Bio-Rad MJ Opticon | |
| for use with Bio-Rad MJ Opticon 2 | |
| for use with Bio-Rad MJ Opticon Cepheid SmartCycler | |
| for use with Eppendorf® Mastercycler ep realplex | |
| for use with Eppendorf® Mastercycler ep realplex2 s | |
| for use with Illumina Eco qPCR | |
| for use with Qiagen Corbett Rotor-Gene 3000 | |
| for use with Qiagen Corbett Rotor-Gene 6000 | |
| for use with Qiagen Corbett Rotor-Gene Q | |
| for use with Roche LightCycler 480 | |
| for use with Strategene Mx3000P | |
| for use with Strategene Mx3005P | |
| for use with Strategene Mx4000 | |
| concentration | 2.5 units/reaction (50 μL reaction volume) |
| detection method | probe-based |
| feature | dNTPs included |
| hotstart | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 20 reactions |
| sufficient for 2000 reactions | |
| sufficient for 400 reactions |
| Application: | JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used: • For quantitative polymerase chain reaction (qPCR) of cDNA template • As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes • For qPCR amplifications using SYBR • For multiplex PCR • For reduction of primer dimers |
| Features and Benefits: | • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield • When performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time and the risk of contamination from multiple pipetting steps • This master mix allows consistent batch-to-batch and reaction-to-reaction performance • Internal Reference Dye is provided for reaction normalization |
| General description: | JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe. To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired. |
| Legal Information: | Eppendorf is a registered trademark of Eppendorf AG |
| Legal Information: | JumpStart is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | Default reaction volume is 50 μL 20RXN is packaged as 1 X 500 μL 400RXN is packaged as 1 X 10 mL 2000RXN is packaged as 1 X 50 mL |
| Preparation Note: | Use 2.5 units per reaction. |
| Symbol |     GHS03,GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H272 - H315 - H317 - H318 - H410 |
| Precautionary statements | P210 - P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xi,N |
| Risk Statements | 43-50/53 |
| Safety Statements | 36/37-61 |
| RIDADR | UN 3082 9 / PGIII |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |