Anti-DSCR1 (C-terminal) antibody produced in rabbit
SIGMA/D6694 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ADAP78; Anti-Calcipressin 1; Anti-DSC1; Anti-Down Syndrome Candidate Region 1; Anti-MCIP1; Anti-
Product Type: Chemical
Immunohistochemistry Anti-DSCR1 (C-terminal): Cat. No. D6694: Immunoperoxidase staining of Anti-DSCR1 (C-terminal) in formalin fixed, paraffin-embedded rat duodenum at 0.2 μg/mL.
Immunohistochemistry Anti-DSCR1 (C-terminal): Cat. No. D6694: Immunoperoxidase staining of Anti-DSCR1 (C-terminal) in formalin fixed, paraffin-embedded rat brain at 0.2 μg/mL.
Immunohistochemistry Anti-DSCR1 (C-terminal): Cat. No. D6694: Immunoperoxidase staining of Anti-DSCR1 (C-terminal) in formalin fixed, paraffin-embedded mouse cecum at 1 μg/mL.
Immunohistochemistry Anti-DSCR1 (C-terminal): Cat. No. D6694: Immunoperoxidase staining of Anti-DSCR1 (C-terminal) in formalin fixed, paraffin-embedded mouse ovary at 1 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human DSCR1 (Lanes 1-3) and HEK293-T non-transfected (Lane 4), were separated on SDS-PAGE, blotted with Anti-DSCR1 (C-terminal) (Cat. No. D6694) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 3 μg/mL 2. Antibody: 1.5 μg/mL 3. Antibody: 3 μg/mL+DSCR1 immunizing peptide (human, 235-252) 4. Antibody: 3 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~26 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2-4 μg/mL using HEK-293T cells expressing human DSCR1 |
| UniProt accession no. | P53805  |
| Application: | Anti-DSCR1 (C-terminal) antibody is suitable for use in western blot (2-4 μg/mL using HEK-293T cells expressing human DSCR1) and in immunoblotting (approx. 26 kDa). |
| Application: | Anti-DSCR1 (C-terminal) antibody produced in rabbit has been used in proximity ligation assay (PLA) and immunoblotting. |
| Biochem/physiol Actions: | Anti-DSCR1 (C-terminal) antibody is specific for DSCR1 in rats, mice and humans. The immunizing peptide specifically inhibits the staining of DSCR1 by immunoblotting. |
| Biochem/physiol Actions: | Down Syndrome Candidate Region 1 (DSCR1) is involved in cellular adaptation to oxidative stress, and interacts with calcineurin to inhibit its phosphatase activity, thereby regulating the calcineurin- Nuclear factor of activated T-cells (NFAT) signaling pathway and affecting central nervous system (CNS) development and angiogenesis. Chronic overexpression of DSCR1 has been implicated in additional pathological conditions including Alzheimer′s disease and cardiac hypertrophy, possibly through inhibition of calcineurin activity. DSCR1 is induced by vascular endothelial growth factor (VEGF) in activated endothelial cells and regulates the expression of inflammatory marker genes such as interleukin-8 (IL-8), tissue factor, E-selectin and cyclooxygenase (Cox-2). |
| Biochem/physiol Actions: | Down Syndrome Candidate Region 1 or DSCR1 is a candidate gene involved in the pathogenesis of Down’s Syndrome (DS). DSCR1 is usually expressed in cardiac, skeletal and brain tissues, whereas its overexpression in fetal brain tissues is associated with DS. DSCR1 inhibits calcineurin-mediated mRNA synthesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DSCR1 (Down Syndrome Candidate Region 1 is encoded by a gene mapped to human chromosome 21q22.1. DSCR1 belongs to a family of conserved proteins named regulators of calcineurin (RCAN), that in humans include RCAN, encoded by the DSCR1L1-2 genes. DSCR1 is highly expressed in the central nervous system (CNS) during early embryonic development, in heart, and in skeletal muscle. |
| Immunogen: | Synthetic peptide corresponding to a sequence at C-terminal of human DSCR1, conjugated to KLH. This sequence is identical in human DSCR1 isoforms a and b, and rat and mouse DSCR1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |