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3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

SIGMA/D6815 - for Membrane Applications

Synonym: DAB membrane substrate

MDL Number: MFCD00012969
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D6815-1SET 1 set
 $493.00
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form liquid
InChI 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
InChI key KJDSORYAHBAGPP-UHFFFAOYSA-N
Quality Level 200 
shipped in wet ice
SMILES string Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
storage temp. 2-8°C
Application: 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.
• in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining
• in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi
• in immunoperoxidase staining of lung tissue
Application: The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.
Biochem/physiol Actions: 3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.
Disclaimer: Stable for up to 18 mo. when stored at 2-8 °C in the original container.
Packaging: Set consists of 4 x 100 ml Solution A and 15 ml Solution B.
Preparation Note: Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.
Storage Temp. 2-8°C
UNSPSC 12352204
Components 3, 3′-Diaminobenzidine enhanced liquid substrate system 4 x 100 mL

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