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2′,7′-Dichlorodihydrofluorescein diacetate

SIGMA/D6883 - ≥97%

Synonym: 2′,7′-Dichlorofluorescin diacetate; DCFH-DA; 2′,7′-Dichlorodihydrofluorescein diacetate

CAS Number: 4091-99-0
Empirical Formula (Hill Notation): C24H16Cl2O7
Molecular Weight: 487.29
MDL Number: MFCD00128955
Linear Formula: C24H16Cl2O7
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D6883-50MG 50 mg
 $55.10
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45-D6883-250MG 250 mg
 $195.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D6883-250MG

 

assay ≥97%
form powder
InChI 1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key PXEZTIWVRVSYOK-UHFFFAOYSA-N
Quality Level 200 
SMILES string CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
storage temp. −20°C
Application: 2′,7′-Dichlorofluorescin diacetate is suitable for use in:
• sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
• microplate assay for detecting oxidative products in phagocytic cells
• quantitative multiwell myeloid differentiation assay
Application: Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.
General description: 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.
General description: The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.
Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research 
Packaging: 50, 250 mg in poly bottle
Preparation Note: This product should be stored in powdered format at -20°C.
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Purity ≥97%
Storage Temp. −20°C
UNSPSC 12352200

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