Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit
SIGMA/D6944 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DAT; Anti-DAT1; Anti-SLC6A3; Anti-Solute carrier family 6, member 3
Product Type: Chemical
Immunofluorescence Whole extract of SH-SY5Y Human neuroblastoma cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 μg/mL Anti-Dopamine Transporter (N-terminal) antibody produced in Rabbit (Cat. No. D6944) followed by 1:200 Anti-Rabbit IgG-Atto 488. Picture A: cells were counterstained with DAPI (blue) to stain nuclei.
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Dopamine Transporter (N-terminal) antibody produced in Rabbit (Cat. No. D6944) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Dopamine Transporter (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 0.5 μg/mL.
Immunohistochemistry Localization of dopamine transporter in rat brain (substantia nigra pars compacta). Formalin-fixed tissue was stained with 1 μg/mL Anti-Dopamine Transporter (N-terminal) (Cat. No. D6944) followed by Donkey Anti-Rabbit IgG, biotin conjugate and by Streptavidin, HRP conjugate (×40 magnification). Image kindly provided by Dr. Shai Shoham, Herzog Hospital, Jerusalem, Israel.
Immunohistochemistry Localization of dopamine transporter in rat brain (ventral tegmental area). Formalin-fixed tissue was stained with 1 μg/mL Anti-Dopamine Transporter (N-terminal) (Cat. No. D6944) followed by Donkey Anti-Rabbit IgG, biotin conjugate and by Streptavidin, HRP conjugate (×40 magnification). Image kindly provided by Dr. Shai Shoham, Herzog Hospital, Jerusalem, Israel.
Immunohistochemistry Localization of dopamine transporter in mouse brain (substantia nigra pars compacta). Formalin-fixed tissue was stained with 1 μg/mL Anti-Dopamine Transporter (N-terminal) (Cat. No. D6944) followed by Donkey Anti-Rabbit IgG, biotin conjugate and by Streptavidin, HRP conjugate (×40 magnification). Image kindly provided by Dr. Shai Shoham, Herzog Hospital, Jerusalem, Israel.
Immunohistochemistry Localization of dopamine transporter in mouse brain (substantia nigra pars compacta). Formalin-fixed tissue was stained with 1 μg/mL Anti-Dopamine Transporter (N-terminal) (Cat. No. D6944) followed by Donkey Anti-Rabbit IgG, biotin conjugate and by Streptavidin, HRP conjugate (×40 magnification). Image kindly provided by Dr. Shai Shoham, Herzog Hospital, Jerusalem, Israel.
Immunoblotting Cell and tissue lysates were separated on SDS-PAGE, probed with 0.5 μg/mL Anti-Dopamine Transporter (N-terminal) (Cat. No. D6944) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK-293T overexpressing human dopamine transporter 2. Rat brain (cytosolic S1 fraction) 3. HEK-293T untransfected 4. Rat brain (cytosolic S1 fraction); Antibody + Dopamine Transporter immunizing peptide (rat, 42-59)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse (predicted), rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 5-10 μg/mL using SH-SY-5Y cells |
| western blot: 0.5-1 μg/mL using rat brain tissue extract | |
| UniProt accession no. | Q01959  |
| Application: | Anti-Dopamine Transporter antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.5-1μg/mL using rat brain extract (cytosolic S1 fraction) and HEK-293T cells expressing human DAT1. It was used as a primary antibody in western analysis for dopamine transporter in several studies. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | DAT regulates dopaminergic-neurotransmi |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Dopamine transporter belongs to a family of biogenic amine transporters that include the norepinephrine, serotonin (SERT), and vesicular monoamine transporters. These transporters are members of a larger Na+/Cl--dependent neurotransmitter transporter family. |
| General description: | Solute carrier family 6 member 3 (SLC6A3) is mapped to human chromosome 5p15.33. It is also known as the dopamine transporter (DAT) and has a zinc binding site and 12 transmembrane region. |
| Immunogen: | synthetic peptide corresponding to amino acids 42-59 of rat dopamine transporter conjugated to KLH. This sequence is highly conserved in mouse DAT (single amino acid substitution) and has lower homology (72% identity) to human DAT. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |