Demecolcine
SIGMA/D7385 - ≥98% (HPLC)
Synonym: N-
CAS Number: 477-30-5
Empirical Formula (Hill Notation): C21H25NO5
Molecular Weight: 371.43
EC Number: 207-514-6
MDL Number: MFCD00075459
Linear Formula: C21H25NO5
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥98% (HPLC) |
| form | powder |
| InChI | 1S/C21H25NO5/c1-22-15-8-6 |
| InChI key | NNJPGOLRFBJNIW-HNNXBMFYSA |
| mp | 184 °C |
| Quality Level | 200  |
| SMILES string | CN[C@H]1CCc2cc(OC)c(OC)c( |
| solubility | chloroform: 10 mg/mL |
| ethanol: soluble | |
| storage temp. | 2-8°C |
| Application: | Demecolcine has been used as a microtubule depolymerizing agent. It has also been used to inhibit mitotic division. |
| Biochem/physiol Actions: | Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase. |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 |
| Precautionary statements | P264 - P301 + P310 |
| Hazard Codes | T |
| Risk Statements | 25 |
| Safety Statements | 22-24/25-45 |
| RIDADR | UN 2811 6.1 / PGII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| mp | 184 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |