MTP™ Taq DNA Polymerase
SIGMA/D7442 - Taq DNA Polymerase, free of DNA contaminants
Synonym: DNA-free Taq DNA polymerase
Product Type: Chemical
Representative QC gel assaying for the presence of conserved 16S rRNA sequence. PCR products run in Lanes 1-3 are from no-template reactions; PCR products in Lanes 4-5, 6-7, and 8-9 are from reactions with 37 fg, 370 fg, and 3.7 pg of E. coli genomic DNA added, Cat. No D4889.
Representative packaging
| color | colorless |
| concentration | 5 unit/μL |
| feature | Difficult Templates/Specialty Enzymes PCR |
| dNTPs included: no | |
| High Fidelity PCR | |
| hotstart: no | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for PCR |
| technique(s) | PCR: suitable |
| usage | sufficient for 1500 reactions |
| sufficient for 250 reactions |
| Application: | MTP™ Taq DNA Polymerase has been used: • for the amplification of bacterial 16S rRNA genes from purified DNA • bacterial genome analysis • pathogen detection |
| Biochem/physiol Actions: | MTP™ Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. |
| Features and Benefits: | • Low contaminant DNA polymerase • Prevents false positive PCR results from contaminating bacterial DNA |
| General description: | MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5′→3′ DNA polymerase and exonuclease activities, is ∼95 kDa by SDS-PAGE, and has no detectable endonuclease or 3′→5′ exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA. Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results, especially when targeting conserved sequences, e.g., bacterial 16S rRNA region. Through Sigma′s proprietary DNA removal methods and strict quality control standards, we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to the conserved region of bacterial 16S rRNA, the Taq expression vector, and the human β-actin gene. While MTP Taq ensures a high-quality, low contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq Buffer with each tube of MTP Taq DNA Polymerase. Each lot of 10× MTP Taq Buffer undergoes the same strict quality control testing as MTP Taq DNA polymerase to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase. |
| Legal Information: | MTP is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Other Notes: | • MT • 10x MTP Taq Buffer (M9943) |
| Other Notes: | Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/spe. |
| Other Notes: | One unit incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74 °C. |
| Other Notes: | Store at –20 °C. For convenience, 10x MTP Taq Buffer can be stored at room temperature. |
| Other Notes: | View more detailed information on MTP Taq DNA Polymerase at www.sigma-aldrich.com/mtp. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |