Anti-Daxx antibody produced in rabbit
SIGMA/D7810 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-BING2; Anti-DAP6; Anti-EAP1; Anti-SMIM40
MDL Number: MFCD01865517
Product Type: Chemical
Western Blotting Whole extract of Jurkat cells were separated on SDS-PAGE and probed with Anti-Daxx antibody produced in Rabbit (Cat. No. D7810). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:4,000 antibody 2. 1:8,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 120 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:4,000 using human acute lymphoma Jurkat whole cell extract | |
| UniProt accession no. | Q9UER7  |
| Application: | Anti-Daxx antibody is suitable for: • western blot (1:4,000 using human acute lymphoma Jurkat whole cell extract) • microarray • the detection and localization of Daxx by immunoblotting |
| Application: | Anti-Daxx antibody produced in rabbit has been used to visualize hDaxx. |
| Biochem/physiol Actions: | Anti-Daxx specifically recognizes human Daxx (120 kDa). |
| Biochem/physiol Actions: | The death domain-associated protein (Daxx) is a protein that interacts with FAS and subsequently regulates cellular apoptosis. Daxx also interacts with H3.3 histone and α-thalassemia X-linked mental retardation protein (ATRX) to regulate chromatin deposition at telomeres. In addition, it also acts as a pro-apoptotic molecule that can specifically enhance Fas-induced apoptosis by activating the Jun N-terminal kinase (JNK) signaling pathway, through its interaction with the apoptosis signal-regulating kinase 1 (ASK1). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Death domain associated protein (Daxx), also known as ETS associated protein 1 (EAP1), is an adaptor protein that binds to the death domain of Fas. Daxx is a 120 kDa protein with a nuclear localization signal sequence. It is highly acidic due to extended glutamic acid-rich domains. Daxx mRNA is extensively expressed in fetal and adult human and mouse tissues. Daxx has also been found in the nucleus where it localizes with the promyelocytic leukemia protein (PML), a gene of acute promyelocytic leukemia (APL). |
| Immunogen: | Synthetic peptide corresponding to the C-terminus of human Daxx. This sequence is identical in mouse and monkey. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |