Diamine Oxidase from porcine kidney
SIGMA/D7876 - ≥0.05 unit/mg solid
Synonym: Amine:oxygen oxidoreductase (deaminating) (pyridoxal-
CAS Number: 9001-53-0
EC Number: 232-613-6
MDL Number: MFCD00130946
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D7876-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D7876-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D7876-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D7876-1G
| biological source | Porcine kidney |
| foreign activity | monoamine oxidase (benzylamine substrate) ≤1% |
| form | solid |
| mol wt | 170 kDa |
| Quality Level | 200  |
| solubility | 100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL |
| specific activity | ≥0.05 unit/mg solid |
| storage temp. | −20°C |
| Application: | Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase. |
| Application: | Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor. |
| Biochem/physiol Actions: | Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide. |
| General description: | Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa. The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4. |
| Other Notes: | One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C. |
| Packaging: | 1, 5, 10 g in poly bottle |
| Packaging: | 250 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.05 unit/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.4.3.22 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |