Dextranase from Penicillium sp.
SIGMA/D8144 - lyophilized powder, 400-800 units/mg protein
Synonym: 1,6-α-D-Glucan 6-glucanohydrolase
CAS Number: 9025-70-1
EC Number: 232-803-9
MDL Number: MFCD00130940
Product Type: Chemical
Dextran is composed of approximately 95% α-D-(1-6) linkages. The remaining α(1-3) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000 dextran molecules exhibit the properties of an expandable coil. At MWs below 2,000 dextran is more rod-like.
| biological source | Penicillium sp. |
| composition | Protein, 35% Lowry |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 400-800 units/mg protein |
| storage temp. | 2-8°C |
| Application: | Dextranase from Penicillium sp has been used: • in the digestion of exopolysaccharides from P. aeruginosa biofilms and dextran polymers • as a component of trypticase soy broth (TSYPS) for Streptococcus mutans culture • in microgel degradation |
| Biochem/physiol Actions: | An endodextranase that hydrolyzes -(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production that increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production. |
| General description: | Dextranase from fungi belong to the glycoside hydrolase families (GH) 49. Dextranase from Penicillium sp. are stable at varying temperature and pH. |
| Other Notes: | One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 500, 1000 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 400-800 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |