Monoclonal Anti-Digoxin antibody produced in mouse
SIGMA/D8156 - clone DI-22, ascites fluid
Synonym: Monoclonal Anti-Digoxin
Product Type: Chemical
ELISA Monoclonal Anti-Digoxin antibody produced in Mouse, Clone: DI-22 (Cat. No. D8156), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) was serially diluted to detect Digoxin by indirect ELISA. Reactivity is observed only with Digoxin-BSA and Digoxin-Transferin but not with BSA and Transferin.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DI-22, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| flow cytometry: suitable | |
| indirect ELISA: 1:10,000 using digoxin-BSA | |
| indirect ELISA: 1:2,500 using digoxigenin-transferrin |
| Application: | Monoclonal Anti-Digoxin antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • dot blot • flow cytometry • fluorescence in situ hybridization (FISH) • DNA hybridization • in-situ hybridization (ISH) • immunodetection of the probe and chromosome X |
| Biochem/physiol Actions: | Antibody is specific for digoxin and shows a high affinity for digoxigenin. |
| Biochem/physiol Actions: | Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive. |
| Biochem/physiol Actions: | Digoxin is a substrate for P-glycoprotein (P-gp), a membrane efflux transporter. This antibody may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides. |
| Biochem/physiol Actions: | Monoclonal Anti-Digoxin antibody is specific for digoxin and digoxin-labeled compounds, and shows strong cross-reactivity with digoxigenin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma DI-22 produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with digoxin- keyhole limpet hemocyanin (KLH). Digoxin is obatained from the leaves of the Digitalis lanata, a foxglove plant. The digoxin molecule is made up of a sugar and a cardenolide. It has a molecular weight is 780.95 Da. It is a odorless white crystal, which is soluble in alcohol and freely soluble in pyridine. It is expressed in the duodenum, kidneys, liver and the blood-brain barrier. |
| Immunogen: | Digoxin-KLH. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |