Monoclonal Anti-Dystrophin antibody produced in mouse
SIGMA/D8168 - clone MANDYS8, ascites fluid
Synonym: Dystrophin Antibody; Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse
MDL Number: MFCD00164586
Product Type: Chemical
Immunohistochemistry Staining of acetone-fixed human tongue frozen sections with 1:400 Monoclonal Anti-Dystrophin Clone: MANDYS8 (Cat. No. D8168) using Goat Anti-Mouse IgG, FITC conjugate.
Immunohistochemistry Staining of acetone-fixed human tongue frozen sections with 1:400 Monoclonal Anti-Dystrophin Clone: MANDYS8 (Cat. No. D8168) using Goat Anti-Mouse IgG, FITC conjugate.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MANDYS8, monoclonal |
| conjugate | unconjugated |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, rat, human, pig, rabbit, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections. | |
| microarray: suitable | |
| western blot: suitable | |
| UniProt accession no. | P11532  |
| Application: | Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin. |
| Application: | Monoclonal Anti-Dystrophin antibody produced in mouse has been used in • immunohistochemistry • immunofluorescence • double immunofluorescence terminal dUTP nick-end labeling (TUNEL) • immunoblotting |
| Biochem/physiol Actions: | Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine. |
| General description: | The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx). |
| Immunogen: | recombinant human dystrophin fragment. |
| Other Notes: | This product can be found as purified product that was produced using cell culture hybridoma product. SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal clone MANDYS8, purified from hybridoma cell culture |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |