Anti-Desmin antibody produced in rabbit
SIGMA/D8281 - whole antiserum
Synonym: Anti-Desmin; Desmin Detection Antibody; Rabbit Anti-Desmin
MDL Number: MFCD00162258
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Human Appendix sections stained with 1:40 Anti-Desmin antibody produced in Rabbit (Cat. No. D8281) (A). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887), and 5 μg/mL Anti-Desmin antibody, Mouse monoclonal, Clone: DE-U-10 (Cat. No. SAB4200707) (B). The antibody was developed using Rabbit Anti-Mouse IgG-Cy3 conjugate antibody. Results Two Anti-Desmin antibodies, D8281 (A) and SAB4200707 (B), target different regions of Desmin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin-fixed, paraffin-embedded heat retrieval tissue section of human tongue stained with 1:20 Anti-Desmin antibody produced in rabbit (Cat. No. D8281) followed by Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat (Cat. No. F9887).
Immunoblotting Chicken gizzard cell extract was separated on SDS-PAGE and probed with Monoclonal AB to Desmin (RB) (Prepack) (Cat. No. D8281). The antibody was developed with HRP APA Rabbit IgG (Gt) (Cat. No. A0545) and an AEC substrate. Antibody dilution 1/200.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | chicken, bovine, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:20 using cultured cells |
| indirect immunofluorescence: 1:20 using human skeletal muscle sections | |
| western blot: 1:100 using animal or human skeletal muscle. | |
| UniProt accession no. | P17661  |
| Application: | Anti-desmin antibody can be used in immunocytochemistry and western immunoblotting. It can also be used in indirect immunofluorescence. |
| Application: | Anti-Desmin antibody produced in rabbit has been used in: • immunohistochemistry of zebrafish intestines sections • immunodetection in fish embryo samples • immunolabeling of myofibers |
| Biochem/physiol Actions: | Desmin plays a crucial role in providing mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Missense mutations in desmin gene leads to hypertrophic cardiomyopathy and systemic myopathy. Mutations in desmin gene impairs the stability of intermediary filaments in cardiomyocytes, contributing to pathophysiology of cardiac diseases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Desmin is an intermediate filament protein found mainly in skeletal, cardiac, and smooth muscle cells. It acts as a marker for tumor origin. It also facilitates mechanical support for muscles, mitochondrial localization, myofibrillogenesis, gene expression regulation, and intracellular signalling. Anti-desmin antibody can be used for immunocytochemical localization of intermediate filaments in all types of muscle cells. It can also be used to stain a wide desmin band of 50,000 to 55,000 molecular weight using immunoblotting assays. Rabbit anti-desmin antibody reacts specifically with desmin in bovine, mouse, chicken and human. |
| General description: | Desmin is mapped to human chromosome 2q35. The desmin protein comprises of a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. In smooth and striated muscles, desmin is localized in the periphery of Z-disk. |
| Immunogen: | desmin from chicken gizzard |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |