REDTaq® Genomic DNA Polymerase
SIGMA/D8312 - with MgCl2
MDL Number: MFCD01635818
Product Type: Chemical
Higher yields from genomic templates with REDTaq Genomic DNA Polymerase.
PCR reactions were set up using 1 μL of mouse genomic DNA and 1 unit of polymerase. The resulting amplicon is a specific 1181 bp fragment. Each sample was prepared in duplicate, and conditions for both sets were identical with the exception of the enzyme used.
Lanes
M. 1 kb DNA marker
1 and 2. REDTaq DNA Polymerase
3 and 4. REDTaq Genomic DNA Polymerase
PCR reactions were set up using 1 μL of mouse genomic DNA and 1 unit of polymerase. The resulting amplicon is a specific 1181 bp fragment. Each sample was prepared in duplicate, and conditions for both sets were identical with the exception of the enzyme used.
Lanes
M. 1 kb DNA marker
1 and 2. REDTaq DNA Polymerase
3 and 4. REDTaq Genomic DNA Polymerase
REDTaq allows quick recognition and confirms appropriate mixing.
Vial 1 contains no REDTaq, vial 2 has 2.5 units of REDTaq added to a 50 μL reaction volume, and vial 3 shows a REDTaq PCR reaction solution thoroughly mixed.
Vial 1 contains no REDTaq, vial 2 has 2.5 units of REDTaq added to a 50 μL reaction volume, and vial 3 shows a REDTaq PCR reaction solution thoroughly mixed.
Representative packaging
| application(s) | agriculture |
| color | red |
| concentration | 1 unit/μL |
| feature | Difficult Templates/Specialty Enzymes PCR |
| dNTPs included: no | |
| hotstart: no | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | sufficient for 1000 reactions |
| sufficient for 250 reactions | |
| sufficient for 2500 reactions |
| Application: | REDTaq® Genomic DNA Polymerase is used for routine PCR amplification of genomic DNA. |
| Features and Benefits: | • Enhanced amplification on genomic and difficult DNA templates • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications • Quick recognition and confirmation of appropriate mixing • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel • The samples can be re-amplified as in nested PCR • The red dye migrates faster than bromophenol blue • Greater consistency across reactions due to proper mixing |
| General description: | REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method. |
| General description: | The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides (the building blocks of DNA). These enzymes are essential for DNA replication and usually work in pairs. It creates two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones. |
| Legal Information: | REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims. |
| Other Notes: | One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |
| Components | 10X PCR Buffer |