Dulbecco’s Phosphate Buffered Saline
SIGMA/D8662 - With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
Synonym: DPBS
MDL Number: MFCD00131855
Product Type: Chemical
| components | sodium pyruvate: no glucose: no (Dextro) |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer. |
| Application: | Dulbecco′s Phosphate Buffered Saline has been used: • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells • to wash cells for protein isolation |
| Application: | Dulbecco′s Phosphate Buffered Saline has been used: • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells • to wash cells for protein isolation |
| Biochem/physiol Actions: | Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions: • provides cells with water and certain bulk inorganic ions essential for normal cell metabolism • serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance • when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism • provides a buffering system to maintain the medium within the physiological pH range |
| General description: | Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |