Deoxyribonuclease II from bovine spleen
SIGMA/D8764 - Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
Synonym: DNase II; Deoxyribonucleate 3′-oligonucleotido-hydrolase
CAS Number: 9025-64-3
EC Number: 232-801-8
MDL Number: MFCD00130919
Product Type: Chemical
Catalyzes the endonucleolytic cleavage of double and single stranded DNA to yield nucleoside 3′-phosphates and 3′-phosphooligonucleotide end-products.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D8764-150KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D8764-30KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D8764-300KU
| application(s) | cell analysis |
| biological source | bovine spleen |
| concentration | ≥50% protein |
| foreign activity | RNase ≤0.1% |
| form | essentially salt-free, lyophilized powder |
| mol wt | 38 kDa |
| Quality Level | 200  |
| specific activity | ≥1,000 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA extraction: suitable |
| tissue culture: suitable | |
| type | Type V |
| Application: | Deoxyribonuclease II from bovine spleen has been used: • as a component of digestion mix to isolate immune cells from colon samples • to digest retinal explants for single-cell suspension preparation • for isolation of stromal cells from human colon tissue • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart |
| Biochem/physiol Actions: | Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiest |
| Other Notes: | One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM |
| Packaging: | 30000, 150000, 300000 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.22.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |