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Anti-Destrin/ADF (GV-13) antibody produced in rabbit

SIGMA/D8815 - affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Actin Depolymerizing Factor

MDL Number: MFCD03791500
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D8815-200UL 200 µL
 $561.00
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Immunohistochemistry Anti-Destrin/ADF (GV-13) Antibody: Cat. No. D8815: Immunoperoxidase staining of Destrin/ADF (GV-13) in formalin fixed, paraffin-embedded rat stomach at 0.02 μg/mL.
Immunohistochemistry Anti-Destrin/ADF (GV-13) Antibody: Cat. No. D8815: Immunoperoxidase staining of Destrin/ADF (GV-13) in formalin fixed, paraffin-embedded mouse heart at 0.02 μg/mL.
Immunohistochemistry Anti-Destrin/ADF (GV-13) Antibody: Cat. No. D8815: Immunoperoxidase staining of Destrin/ADF (GV-13) in formalin fixed, paraffin-embedded mouse brain at 0.02 μg/mL.
Western Blotting Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Destrin/ADF (GV-13) antibody produced in Rabbit (Cat. No. D8815). The antibody was developed using 1:6,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:1,500 antibody 2. 1:3,000 antibody 3. Negative Control

 

antibody form affinity isolated antibody
antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration ~0.5-1.0 mg/mL
conjugate unconjugated
form buffered aqueous solution
Quality Level 200 
shipped in dry ice
species reactivity human, canine, mouse, rat
storage temp. −20°C
target post-translational modification unmodified
technique(s) indirect immunofluorescence: 1:100 using mouse NIH/3T3 fibroblasts
  western blot: 1:1,500 using whole extracts of human A-431 epidermoid carcinoma cells, rat PC-12 pheochromocytoma cells, and dog MDCK kidney cells
UniProt accession no. P60981 
Application: Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:1500 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells and for indirect immunofluorescence at a working dilution of 1:100 using mouse NIH/3T3 fibroblasts. It was used in a study to label chick neurons treated with antimycin to examine the colocalization of actin, cofilin, and ADF in pMAP (phosphorylated microtubule-associated protein) inclusions after ATP depletion. It was used to specifically detect ADF in a study.
Biochem/physiol Actions: Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca2+ independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.
Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description: Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘Hirano bodies’ in certain brain neurons of dementia patients.
Immunogen: synthetic peptide corresponding to amino acids 153-165 of human destrin/ADF.
Physical form: Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.
WGK Germany -
Storage Temp. −20°C
UNSPSC 12352203

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