Deoxyribonucleic acid, single stranded from calf thymus
SIGMA/D8899 - lyophilized powder
Synonym: DNA single stranded
CAS Number: 91080-16-9
EC Number: 293-507-3
MDL Number: MFCD00163436
Product Type: Chemical
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| biological source | bovine (calf) thymus |
| composition | ssDNA, ≥65% |
| form | lyophilized powder |
| grade | Molecular Biology |
| mol wt | ~50 kb |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. |
| Application: | Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay. |
| Features and Benefits: | • Activated • No nuclease activity detected after 16 h at 37 °C incubation • High quality template DNA |
| General description: | High quality single-stranded template DNA isolated from the thymus of male and female calves. |
| Preparation Note: | Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water. |
| Preparation Note: | One mg DNA is approx. 25 A260 units. |
| Preparation Note: | Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106310 |