Anti-Destrin/ADF (AE-14) antibody produced in rabbit
SIGMA/D8940 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Actin Depolymerizing Factor
MDL Number: MFCD03791499
Product Type: Chemical
Western Blotting Whole cell extract of NIH-3T3 cells were separated on SDS-PAGE and blotted with Anti-Destrin/ADF (AE-14) antibody produced in Rabbit (Cat. No. D8940). The antibody was developed with 1:10,000 Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545). Lanes 1. 1:600 antibody 2. 1:1,200 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~0.8-1.2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:600 using whole extracts of human A431 epidermoid carcinoma cells, rat PC-12 pheochromocytoma cells, or mouse NIH3T3 fibroblasts | |
| UniProt accession no. | P60981  |
| Application: | Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:600 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and mouse NIH/3T3 fibroblasts, and a chemiluminescent detection reagent. |
| Biochem/physiol Actions: | Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitiv |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Destrin/ADF and cofilin are widely distributed in tissues of eukaryotes and both contain a nuclear localization sequence. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. This gene is located on human chromosome 20p12. |
| General description: | Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘Hirano bodies’ in certain brain neurons of dementia patients. |
| Immunogen: | synthetic peptide corresponding to amino acids 137-150 of human destrin/ADF. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |