o-Dianisidine dihydrochloride
SIGMA/D9154 - tablet, 10 mg substrate per tablet
Synonym: 3,3′-Dimethoxybenzidine dihydrochloride; Fast Blue B
CAS Number: 20325-40-0
Empirical Formula (Hill Notation): C14H16N2O2 · 2HCl
Molecular Weight: 317.21
EC Number: 243-737-5
MDL Number: MFCD00012488
Linear Formula: C14H16N2O2 · 2HCl
Product Type: Chemical
| form | tablet |
| InChI | 1S/C14H16N2O2.2ClH/c1-17- |
| InChI key | UXTIAFYTYOEQHV-UHFFFAOYSA |
| Quality Level | 200 ![]() |
| SMILES string | Cl.Cl.COc1cc(ccc1N)-c2ccc |
| solubility | water: 1 tablet/10 mL, clear, colorless |
| storage temp. | 2-8°C |
| Biochem/physiol Actions: | o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl. |
| Disclaimer: | Probably carcinogenic. |
| Packaging: | 50, 100 tabs in glass bottle |
| Preparation Note: | Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use. |
| Symbol | ![]() GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H350 |
| Precautionary statements | P201 - P301 + P312 + P330 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 45-22 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |



