Dimethyl sulfoxide
SIGMA/D9170 - PCR Reagent
Synonym: DMSO
CAS Number: 67-68-5
Empirical Formula (Hill Notation): C2H6OS
Molecular Weight: 78.13
EC Number: 200-664-3
MDL Number: MFCD00002089
Linear Formula: (CH3)2SO
Product Type: Chemical
| application(s) | agriculture |
| assay | ≥99.5% |
| autoignition temp. | 573 °F |
| bp | 189 °C (lit.) |
| color | colorless |
| density | 1.10 g/mL (lit.) |
| expl. lim. | 42 %, 63 °F |
| form | liquid |
| impurities | ≤0.1% water water |
| InChI | 1S/C2H6OS/c1-4(2)3/h1-2H3 |
| InChI key | IAZDPXIOMUYVGZ-UHFFFAOYSA |
| mp | 16-19 °C (lit.) |
| packaging | vial of 1 mL |
| Quality Level | 200  |
| refractive index | n |
| shelf life | Recommended retest period - 2 years |
| SMILES string | CS(C)=O |
| solubility | H2O: miscible (completely) |
| suitability | suitable for PCR |
| technique(s) | PCR: suitable |
| vapor density | 2.7 (vs air) |
| vapor pressure | 0.42 mmHg ( 20 °C) |
| Application: | Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture and as PCR facilitator. |
| Disclaimer: | Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product. |
| General description: | Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs). Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR. |
| Other Notes: | For information on dimethyl sulfoxide miscibility, please visit the following link: Dimethyl Sulfoxide Miscibility/Immiscibility Table |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | 188.6 °F - closed cup |
| Flash Point(C) | 87 °C - closed cup |
| Purity | ≥99.5% |
| bp | 189 °C (lit.) |
| mp | 16-19 °C (lit.) |
| Density | 1.10 g/mL (lit.) |
| Refractive Index | n |
| UNSPSC | 12191502 |