Lambda DNA EcoR I Hind III Digest
SIGMA/D9281 - for DNA electrophoresis
Product Type: Chemical
Representative product in the bag
Representative product tube
| form | liquid |
| Quality Level | 100  |
| 200 |
|
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | 100 uses (0.1 mg size) |
| 500 uses (0.5 mg size) |
| Application: | Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products. |
| Application: | Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. |
| General description: | Sigma’s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
| Other Notes: | Contains 13 fragments, 125–21,226 bp |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 0.7%. |
| Other Notes: | Sigma’s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA. |
| Preparation Note: | Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Codes | Xi |
| Risk Statements | 43 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41141827 |