DNA Polymerase I from Escherichia coli lysogenic for NM 964
SIGMA/D9380 - buffered aqueous glycerol solution
Synonym: Kornberg Polymerase
CAS Number: 9012-90-2
MDL Number: MFCD00130924
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| concentration | 5,000-15,000 units/mL |
| foreign activity | Endonuclease, none detected |
| form | buffered aqueous glycerol solution |
| grade | Molecular Biology |
| mol wt | 109 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P00582  |
| Application: | DNA Polymerase I from Escherichia coli has been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity. |
| Application: | Suitable for: • Highly specific DNA probes by nick translation • In vitro synthesis of complementary cDNA strand • In vitro synthesis of DNA • Produce blunt ends from 5′ and 3′ overhangs |
| General description: | DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced. |
| Other Notes: | DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol. |
| Other Notes: | One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |