pBR322 Hae III Digest
SIGMA/D9655 - for DNA electrophoresis
Product Type: Chemical
DÂ 9655 on a 20% PAGE gel
Representative product tube
Representative product in the bag
| form | liquid |
| Quality Level | 100  |
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | 1000 uses (0.5 mg size) |
| 200 uses (0.1 mg size) |
| Application: | Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. |
| General description: | Sigma’s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
| Other Notes: | Contains 22 fragments, 8–587 bp |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 2.5. |
| Other Notes: | Sigma’s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA. |
| Preparation Note: | Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |