Anti-Doublecortin antibody produced in rabbit
SIGMA/D9693 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DCX; Anti-Doublecortex; Anti-LISX; Anti-X-linked lissencephaly; Anti-XLIS
MDL Number: MFCD03454961
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Doublecortin antibody produced in Rabbit (Cat. No. D9693) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Human Doublecortin (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:3,000.
Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-Doublecortin (Cat. No. D9693). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:3,000 Lanes 1,5: Rat brain (S1 fraction) 2: Mouse brain (S1 fraction) 3: HEK-293T cells ransfected with human Doublecortin 4: Untransfected HEK-293T cells 5: Inhibition with Doublecortin immunizing peptide (human, 151-170) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~40 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cells expressing human doublecortin. Also, mouse and rat brain extract (S1 fraction). |
| UniProt accession no. | O43602  |
| Application: | Anti-Doublecortin antibody produced in rabbit has been used in immunoblotting and immunohistofluorescence. |
| Application: | Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2μg/mL using HEK-293T cells expressing human doublecortin, and mouse brain and rat brain extract (S1 fraction). |
| Biochem/physiol Actions: | Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide corresponding to amino acids 151-170 of human doublecortin, conjugated to KLH. The corresponding sequence is identical in rat and mouse doublecortin. |
| Physical form: | Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |