Lambda DNA Hind III Digest
SIGMA/D9780 - for DNA electrophoresis
Synonym: Hind III digested bacteria phage Lambda DNA; Hind III digested bacteria phage Lambda DNA
Product Type: Chemical
| form | liquid |
| grade | Molecular Biology |
| Quality Level | 200 ![]() |
| shipped in | dry ice |
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | 100 uses (0.1 mg size) |
| 1000 uses (1 mg size) | |
| 20 uses (20 μg size) | |
| 500 uses (0.5 mg size) |
| Application: | Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. |
| General description: | Sigma’s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
| Other Notes: | Contains 8 fragments, 125–23,130 bp |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 0.75%. |
| Other Notes: | Sigma’s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA. |
| Preparation Note: | Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41141827 |

