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DMEM/Nutrient Mixture F-12 Ham

SIGMA/D9785 - With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Synonym: Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham; DME/F-12, 1:1 mixture

MDL Number: MFCD00217417
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D9785-10L 10 L
 $142.00
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components L-glutamine: no
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
NaHCO3: no
phenol red: no
glucose: 3.15 g/L
form powder
shipped in ambient
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
General description: The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.
Preparation Note: Formulated to contain 14.8 grams of powder per liter of medium.
Preparation Note: Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352207

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