Denhardt’s Solution
SIGMA/D9905 - for Northern and Southern blotting, lyophilized powder
Synonym: Denhardt′s Solution For Blotting Technique; Denhardt′s Solution For Blotting Technique
Product Type: Chemical
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| foreign activity | DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected |
| form | lyophilized powder |
| grade | Molecular Biology |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | Southern blotting: suitable |
| Application: | Denhardt′s Solution has been used: • as a component in hybridization buffer for the hybridization of brain sections • in in-situ hybridization peroxidase-(antiperoxidas • in the preparation of hybridization mixture |
| Application: | Suitable as a blocking Buffer for Southern and Northern blot membranes. |
| Biochem/physiol Actions: | Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization. |
| General description: | Denhardt’s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers. |
| Packaging: | 5 mL in glass bottle |
| Preparation Note: | Each vial of Denhardt’s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP). |
| Preparation Note: | To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161700 |