Anti-Doublecortin (N-terminal) antibody produced in rabbit
SIGMA/D9943 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DCX; Anti-Doublecortex; Anti-LISX; Anti-X-linked lissencephaly; Anti-XLIS
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Doublecortin (N-terminal) antibody produced in Rabbit (Cat. No. D9943) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Doublecortin (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:3,000.
Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-Doublecortin (N-terminal) (Cat. No. D9943). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:3,000 Lanes 1,5: Rat brain (S1 fraction) 2: Mouse brain (S1 fraction) 3: HEK-293T cells transfected with human Doublecortin 4: Untransfected HEK-293T cells 5: Inhibition with Doublecortin immunizing peptide (rat, 1-17) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~40 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction) |
| western blot: 1-2 μg/mL using mouse brain extract (S1 fraction) | |
| UniProt accession no. | O43602  |
| Application: | Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction), and 1-2μg/mL using mouse brain extract (S1 fraction). |
| Biochem/physiol Actions: | Anti-Doublecortin(N-termi |
| Biochem/physiol Actions: | Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Doublecortin gene is mapped to human chromosome Xq23. It comprises two homologous doublecortin (DC) domains, one each in the N-terminal and C-terminal region. A linker connects a flexible unstructured region. The C-terminal sequences also have serine/proline-rich region. |
| Immunogen: | synthetic peptide corresponding to amino acids 1-17 located at the N-terminus of rat doublecortin, conjugated to KLH. This sequence is identical in mouse, and highly conserved in human doublecortin. |
| Physical form: | Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |