Anti-DOCK1 (C-terminal) antibody produced in rabbit
SIGMA/D9945 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DOCK180; Anti-Dedicator of cytokinesis 1; Anti-Downstream of CRK-binding protein, 180-KD
Product Type: Chemical
Immunofluorescence Cytoplasmic localization of DOCK1 in NIH3T3 cells. Fixed cells were stained with 10 μg/mL Anti-DOCK1 (C-terminal) (Cat. No. D9945) followed by Goat anti-Rabbit IgG, Cy3 conjugate (x40 magnification). Nuclear staining with DAP1.
Immunoblotting Cell lysates were separated on SDS-PAGE, blotted with 2 μg/mL Anti-DOCK1 (C-terminal) (Cat. No. D9945) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK293-T 2. PC12 3. HeLa 4. NIH3T3 5. PC12. Antibody+DOCK1 immunizing peptide (human, 1851-1865)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~180 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10-20 μg/mL using NIH-3T3 cells |
| western blot: 2-4 μg/mL using HeLa or PC12 cell lysates | |
| UniProt accession no. | Q14185  |
| Application: | Anti-DOCK1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Anti-DOCK1 (C-terminal) specifically recognizes human, rat, and mouse DOCK1. |
| Biochem/physiol Actions: | Dedicator of cytokinesis protein 1 (DOCK1) is involved in cytoskeletal reorganization and cell migration and acts as an activator of Rac. DOCK1 has been found to undergo ubiquitination and proteasome-dependent degradation, a process enhanced in response to epidermal growth factor (EGF), adapter molecule (Crk) and adhesion-dependent signals. DOCK1 forms a complex with engulfment and cell motility protein 1 (ELMO1) which also inhibits ubiquitylation of DOCK1. By activating the β1 and αvβ5 integrins, DOCK1 regulates cell motility, filopodia formation, and phagocytosis. DOCK1 has been suggested to play a central role in netrin signaling during neuronal development by mediating the attractive responses by neurons to netrin-1. It mediates morphological changes such as integrin-mediated cell spreading and nerve growth factor (NGF)-induced neurite outgrowth. In mammalian cells, the CrkII-ELMO1-DOCK1 complex has been shown to promote phagocytosis. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Dedicator of cytokinesis protein 1 (DOCK1), also termed as the DOCK180 gene, is mapped to human chromosome 10q26.2. DOCK1 is a member of a family of guanine nucleotide exchange factors (GEF). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| WGK Germany | 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |