DEAE–Sepharose™
SIGMA/DFF100 - Fast Flow
Synonym: Diethylaminoethyl–Sepharose™
CAS Number: 57407-08-6
MDL Number: MFCD00146630
Product Type: Chemical
| bead size | 45-165 μm (wet) |
| capacity | 110-160 μeq/mL binding capacity(gel volume) |
| form | suspension |
| matrix | 6% cross-linked agarose |
| pH | 2—12 |
| pore size | ~4,000,000 Da exclusion limit |
| Quality Level | 200 ![]() |
| technique(s) | affinity chromatography: suitable |
| Application: | DEAE-Sepharose has been used in: • anion exchange chromatography • to screen polyisoprenyl phosphate phosphatase activity • to purify isoinhibitors • for the purification of human immunodeficiency virus (HIV) • glycoprotein envelope (gp140 env) |
| Application: | DEAE-Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile. |
| Biochem/physiol Actions: | Diethylaminoethyl-sepharo |
| General description: | DFF100-500Ml′s update product number is GE17-0709-01 |
| Legal Information: | Sepharose is a trademark of Cytiva |
| Packaging: | 50, 100, 500 mL in glass bottle |
| Symbol | GHS02 |
| Signal word | Warning |
| Hazard statements | H226 |
| Precautionary statements | P210 - P233 - P240 - P241 - P242 - P243 |
| Risk Statements | 10 |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | 100.4 - 109.4 °F |
| Flash Point(C) | 38 - 43 °C |
| UNSPSC | 47101511 |


