DNA Quantitation Kit, Fluorescence Assay
SIGMA/DNAQF - Quantitation of DNA using bisBenzimide
Synonym: dsDNA quantification
Product Type: Chemical
DNA quantitation assay with bisBenzimide H 33258 (Hoechst 33258). When excited at 360em/460ex, the dye increases significantly in the presence of DNA.
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | nucleic acid detection: suitable |
| usage | sufficient for 750 assays (2 ml each) |
| Application: | DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA: • in neonatal rat ventricular myocytes (NRVMs) • in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount • in mouse embryonic fibroblast cell line (NIH3T3 cells) |
| Features and Benefits: | • Specific for the quantitation of nanogram amounts of DNA • Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins |
| General description: | The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 41105500 |
| Components | Detailed technical bulletin 1 ea |