Duolink® In Situ Microplate Nuclear Stain, Anti-Fade
SIGMA/DUO82064
Synonym: in situ Proximity Ligation Assay reagent; Protein Protein Interaction Assay reagent
Product Type: Chemical
Duolink® PLA Workflow
| fluorescence | λex 360 nm; λem 460 nm |
| product line | Duolink® |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| suitability | suitable for fluorescence-detection automated sequencing |
| suitable for microtiter plates | |
| technique(s) | proximity ligation assay: suitable |
| Application: | Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples. Use the Multiwell Plates modifications to the Duolink® In Situ Fluorescence Protocol to run an experiment with this product. A set of short instructions can also be used.Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment. |
| Application: | Specificity Duolink® In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used after staining cells with Duolink® In Situ in microtiter plates. See the datasheet for more information. Application Note Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required. Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projectsView full Duolink® product list |
| Features and Benefits: | • No overexpression or genetic manipulation required • High specificity (fewer false positives) • Single molecule sensitivity due to rolling circle amplification • Relative quantification possible • No special equipment needed • Quicker and simpler than FRET • Increased accuracy compared to co-IP • Publication-ready results |
| Legal Information: | Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | PLA is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H317 - H411 |
| Precautionary statements | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| Hazard Codes | N |
| Risk Statements | 51/53 |
| Safety Statements | 61 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |