Duolink® In Situ PLA® Probe Anti-Rabbit MINUS
SIGMA/DUO92005
Synonym: in situ Proximity Ligation Assay reagent; Protein Protein Interaction Assay reagent
Product Type: Chemical
Duolink PLA Probe representative packaging.
Duolink® PLA Workflow
| antibody form | affinity purified immunoglobulin (secondary antibody) |
| antibody product type | primary antibodies |
| biological source | donkey (polyclonal) |
| product line | Duolink® |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | rabbit |
| storage temp. | 2-8°C |
| suitability | suitable for brightfield |
| suitable for fluorescence | |
| technique(s) | immunofluorescence: suitable |
| proximity ligation assay: suitable |
| Application: | Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples. This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection. |
| Application: | Specificity PLA probe anti-Rabbit reacts with whole molecule rabbit IgG and the light chains of other rabbit immunoglobulin?s. The PLA probe anti-Rabbit has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.Application Note Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required. Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projectsView full Duolink® product list |
| Features and Benefits: | • No overexpression or genetic manipulation required • High specificity (fewer false positives) • Single molecule sensitivity due to rolling circle amplification • Relative quantification possible • No special equipment needed • Quicker and simpler than FRET • Increased accuracy compared to co-IP • Publication-ready results |
| Legal Information: | Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | PLA is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | This product is comprised of the following: • 5x PLA Probe Anti-Rabbit MINUS - Donkey anti-rabbit secondary antibody conjugated to oligonucleotide MINUS • 1x Blocking Solution - Reagent for blocking of the sample • 1x Antibody Diluent - For dilution of PLA probes and primary antibodies See datasheet for more information. |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |