Duolink® In Situ Orange Starter Kit Mouse/Rabbit
SIGMA/DUO92102
Synonym: in situ Proximity Ligation Assay reagent; Protein Protein Interaction Assay reagent
Product Type: Chemical
The Duolink PLA Starter Kits supply all the necessary reagents – excluding primary antibodies – to run a Duolink PLA assay, representative packaging.
Duolink PLA orange fluorescent detection.
Duolink® PLA Workflow
| fluorescence | λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20) |
| product line | Duolink® |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| technique(s) | proximity ligation assay: suitable |
| Application: | Duolink®proximity ligation assay(PLA®)  allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructions is also available.Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment. |
| Application: | Specificity The Duolink® In Situ Orange Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Orange fluorescence detection reagents are often used with Cyanine 3 filter. Application Note Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required. Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projectsView full Duolink® product list |
| Application: | Duolink® In Situ Orange Starter Kit Mouse/Rabbit has been used in in situ interaction assay. |
| Features and Benefits: | • No overexpression or genetic manipulation required • High specificity (fewer false positives) • Single molecule sensitivity due to rolling circle amplification • Relative quantification possible • No special equipment needed • Quicker and simpler than FRET • Increased accuracy compared to co-IP • Publication-ready results |
| Legal Information: | Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | PLA is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Symbol |   GHS05,GHS09 |
| Signal word | Warning |
| Hazard statements | H290 - H411 |
| Precautionary statements | P234 - P273 - P390 - P391 - P501 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 41116158 |
| Components | Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L); Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L); Duolink® In Situ Detection Reagents Orange; Duolink® In Situ Wash Buffers, Fluorescence; Duolink® In Situ Mounting Medium with DAPI |