Duolink® flowPLA Detection Kit - Red
SIGMA/DUO94001 - DUOLINK® : PLA kit for Flow Cytometry with Red Detection
Synonym: in situ Proximity Ligation Assay; Flowcytometry-PLA; Protein Protein Interaction Kit
Product Type: Chemical
Flow cytometry workflow using Duolink® PLA is a powerful tool for protein-protein interaction detection, protein translational modification detection and protein expression experiments.
Duolink PLA Detection Reagents representative packaging.
| fluorescence | λex 594 nm; λem 624 nm |
| product line | Duolink® |
| shipped in | dry ice |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| technique(s) | flow cytometry: suitable |
| immunofluorescence: suitable | |
| proximity ligation assay: suitable |
| Application: | Application Note Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended. Let us do the work for you, learn more about our Custom Service Program  to accelerate your Duolink® projectsView full Duolink® product list |
| Application: | Based on proximity ligation assay (PLA), the Duolink® PLA Technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells. Duolink® flowPLA Detection Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest, a pair of PLA probes (one 100RXN PLUS and one 100RXN MINUS), wash buffer, and a Duolink® flowPLA Detection Kit. The flowPLA Kits are available with 5 different fluorophores: Violet, Red, Green, Orange, or FarRed. The flowPLA Kits contain all the necessary reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, primary antibodies, and corresponding PLA Probes. Follow the Duolink® PLA Flow Cytometry Protocol to use this product.Visit our Duolink® PLA Flow Cytometry page on how to run a Duolink® flow experiment, applications, troubleshooting, and more. |
| Biochem/physiol Actions: | Red Fluorescence Detection Reagents Use appropriate laser for λex 594 nm excitation Use appropriate filter for λem 624 nm emission |
| Features and Benefits: | • Analyze protein protein interactions with flow cytometry readout • Analyze cell populations with Proximity Ligation Assay • Increased sensitivity due to rolling circle amplification for low abundant targets • No overexpression or genetic manipulation required • Relative quantification possible • Works with any flow cytometer instrumentation • Easy to follow flexible protocol • Publication-ready results |
| Legal Information: | Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | PLA is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | This product is comprised of the following: • 5x Detection Solution - Red (DUO84001) • 5x Ligation Buffer (DUO82009) • 5x Amplification Buffer (DUO82050) • Ligase (1U/μL) • Polymerase (10U/μL) See datasheet for more information. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | −20°C |
| UNSPSC | 41105331 |