Anti-eIF2α antibody produced in rabbit
SIGMA/E0157 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-EIF2; Anti-EIF2S1; Anti-Eukaryotic translation initiation factor 2, subunit 1; Anti-eIF-2A
Product Type: Chemical
Immunohistochemistry (Paraffin) Formalin Fixed Paraffin Embedded (FFPE) human cerebral cortex (A) and Negative control (no primary) (B) tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:250 dilution of Cat. No. E0157, Anti-eIF2α antibody. Reactivity was detected using a Goat Anti-Rabbit IgG and HRP-DAB. Cytoplasmic/membranous staining was observed in neurons of human cerebral cortex tissue sections.
Immunoblotting PC12 lysate was separated on SDS-PAGE and probed with Rabbit Anti-eIF2a (Cat. No. E0157). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Antibody dilution 1:1,000 3. Antibody dilution 1:500 + 20 μg/mL eIF2α immunizing peptide 4. Antibody dilution 1:500 + 20 μg/mL nonrelevant peptide
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~35 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:250 using human cerebral cortex sections |
| western blot: 1:250-1:500 using PC-12 cell lysate | |
| UniProt accession no. | P05198  |
| Application: | Anti-eIF2α antibody produced in rabbit has been used in western blotting. Anti-elF2α antibody is also suitable for immunohistochemistry at a working dilution of 1:250 using human cerebral cortex sections. |
| Application: | Anti-eIF2α antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:250-1:500 using PC-12 cell lysate. It was used as a primary antibody to detect eIF2α in a study. |
| Biochem/physiol Actions: | Anti-eIF2α (also known as eIF-2A) recognizes human eIF2α. |
| Biochem/physiol Actions: | Increased eIF2α phosphorylation has been observed in Alzheimer′s disease. Viral infections are also known to induce eIF2α phosphorylation. |
| Biochem/physiol Actions: | The main role of eIF2 complex is to transfer Met-tRNAi in the presence of GTP to the 40S ribosomal subunit during the initiation of translation. Regulation of the initiation of translation at the level of eIF2 involves the phosphorylation of eIF2α at a conserved Ser51 residue. This leads to inhibition of eIF2β activity, thus reducing guanine nucleotide exchange and consequently, the translation rate. Protein synthesis inhibition at the level of eIF2α phosphorylation is linked to a variety of cell stress signaling pathways via specific kinases. Phosphorylation of eIF2α reduces the rate of translation and gives the cell time to correct the stress damage, and selectively enhance the translation of genes important for stress remedy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The eukaryotic translation initiation factor 2 subunit |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |