Elastase from porcine pancreas
SIGMA/E0258 - Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)
Synonym: Elastase from hog pancreas; Pancreatopeptidase E
CAS Number: 39445-21-1
EC Number: 254-453-6
MDL Number: MFCD00130998
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E0258-10MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E0258-20MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E0258-50MG
| biological source | Porcine pancreas |
| composition | Protein, 50-90% |
| foreign activity | trypsin ≤50 BAEE units/mg protein |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | ≥4.0 units/mg protein (biuret) |
| storage temp. | −20°C |
| type | Type IV |
| Application: | Elastase from porcine pancreas has been used in a study to assess the molecular bases for human leucocyte elastase inhibition. Elastase from porcine pancreas has also been used in a study to investigate the molecular cloning and expression of serum calcium-decreasing factor (caldecrin). |
| Application: | Elastase from porcine pancreas has been used: • to treat vero cells to study its effects on syncytium formation • as a positive control in protease assays • as a component in RPMI 1640 to isolate human aortic smooth muscle cells (HASMCs) from the aortic tissue |
| Application: | Elastase from Sigma has been used to examine the extent of proteolytic degradation of BSA that is treated with hydroxyl radical. It has also been used to purify elastase-inhibitory lipid derivative from a cyanobacterium, Microcystis Ku2. |
| Biochem/physiol Actions: | Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin. |
| Biochem/physiol Actions: | Elastase is a serine protease with broad specificity as it cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells. Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin. |
| Biochem/physiol Actions: | Elastase is a serine protease with broad specificity as it cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells. |
| General description: | Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of N-succinyl- |
| Packaging: | 1, 5, 10, 20, 50 mg in glass bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Contains sodium carbonate. |
| Preparation Note: | A further purification of Type III, E 0127, by affinity chromatography to reduce trypsin activity |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4.0 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.36 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |