Monoclonal Anti-EDEM3 antibody produced in mouse
SIGMA/E0409 - ~1.0 mg/mL, clone EDEM3-1, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-ER degradation enhancer, mannosidase α-like 3
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-EDEM3 Clone: EDEM3-1 (Cat. No. E0409). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T cells; 2. HEK-293T cells overexpressing human EDEM3; 3. HeLa; 4. HEPG2; 5. MCF7; 6. NRK; 7. L8; 8. 3T3; 9. C2C12
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | EDEM3-1, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~120 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using whole extract of mouse 3T3 or rat NRK cells |
| UniProt accession no. | Q9BZQ6  |
| Application: | Monoclonal Anti-EDEM3 antibody produced in mouse has been used in immunoblotting. |
| Biochem/physiol Actions: | EDEM3 (ER degradation enhancer, mannosidase α-like 3), a soluble EDEM homolog, enhances glycoprotein endoplasmic reticulum-associated degradation (ERAD) and mannose trimming. EDEM3 accelerates ERAD of misfolded glycoproteins as well, but in ontrast to EDEM1, it greatly stimulates mannosidase trimming in vivo. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-EDEM3 (mouse IgG1 isotype) is derived from the hybridoma EDEM3-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EDEM3 conjugated to KLH. |
| General description: | Three EDEM homologs, EDEM1, EDEM2 and EDEM3 have been identified, which are transcriptionally upregulated upon ER stress by the activated IRE1/Xbp-1 branch. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| WGK Germany | 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |